Hemagglutinin

 Hemagglutinins (HA) are one of the two antigenic Glycoproteins inserted into the influenza virus' membrane. There are at least 16 different forms of HA Antigens classified from H1 to H16. H1, H2, H3 are specific to human influenza. HAs have two main functions crucial for the viral infection cycle: 1. On the target cell, HA bind the receptor which is composed by Sialic acid allowing the virus/cell interaction. 2. HA induce the fusion between the host cell and the virus which can entry into the cytoplasm. Because the HA are the major Antigens of the Virus, Antibodies recognized them and for this reason HA often change.

For discussion of influenza hemagglutinin see Influenza hemagglutinin.

Hemagglutinin Structure
HA is an homotrimer integral membrane glycoprotein. Each monomer is synthesized like a single polypeptide chain almost 550 amino acids. This precursor is then glycosilated and cleaved into two smaller polypeptides by removal of Arginin 329; at the same time, a conformational change occurs in the monomer.

The HA1 and HA2 subunits are covalently attached by a disulfide bond from HA1 position 14 to HA2 position 467(*). These two chains form one monomer, and the noncovalentely association of three monomers forms one hemagglutinin molecule: (HA1+HA2)3. It is principally stabilized by packing of the alpha-helixes. All molecules are 135 Angström long.

The HA1 subunit (328 amino acids) has a globular form outside the virus’ membrane: it is composed by an eight-stranded beta-sheet, associated with a little alpha-helix, separating the strands 3 and 4. The amino acids of this alpha-helix, and some others around it, included in the beta-sheet, compose the binding site for the receptor’s sialic acid. Thus, for one molecule of hemagglutinin, there are three binding sites to the host cell’s receptor.

The HA2 subunit (221 amino acids) has a hair spin structure composed by two antiparallel alpha-helixes. One of these belongs to the longest alpha-helixes in globular protein: it is 50 angstrom long. The hydrophobic N-terminus part of HA2, called fusion peptide, is close to a protease’s cleavage site: this protease is implied in the virus’ entry in the host cell.

HA1 and HA2 are bound each other by a disulfide bind. The three long alpha-helixes (of the three HA2) are coiled-coil to form a central region of 40 Angström, and thank to the hydrophobic amino acids and those which form salt bridges bound, we obtain a HA stabilized.

2WRE
To begin, this code corresponds to the structure of influenza H2 Avian Hemagglutinin from the “Asian Influenza” of 1957. Human receptor. -	At the position 226, Avian Hemagglutinin has a Glutamine residue whereas the Human one has Leucine

-	At the position 228, there is a Glycin for Avian HA whereas a Serine for Human HA.

These two mutations prevent the human H2 binding on the avian receptor whereas the human receptor can be bound by avian hemagglutinin that lacks the human specific mutation of H2 pandemic viruses.

There are three sites for sialic acid binding, which are at the membrane-distal tips of the identical monomers that form the HA trimer:

-  The first one is the Loop 220 composed by the residues from 225 to 228

-  The second is called Loop 130 and contains the residues 131 to 137

-  the last is named 190-helix

The residues of these loops 130 and 220 have carbonyl oxygens and amide nitrogens of peptide bonds exposed with potential to interact with the receptor, which is composed by Gal-1 and Sia-2. The avian H2 can bind the human receptor because of intramolecular Hydrogen bond network created by Gln 226 and by Asn 186. Four amino acids compose the receptor binding site : Tyr 98, Ser 136, Trp 153(**), His 183, (which are identical in the all different HA)(*). This site forms a pocket on the distal end of the molecule. The binding structure is stabilized by other conserved which cannot interact with the receptor (Cys 97, Pro 99, Cis139, Pro 147, Tyr 195, Arg 229). (*) The Sia-1-Gal-2 glycosidic bond adopts a cis conformation. Extensive hydrogen bond direction are settled between avian and Gal-2 of the receptor: two water molecules (Wat-1 and Wat-2) have a significant role in mediating these interactions(see fig.1). The site chain of Lys-222 and the main chain carbonyl at 225 are linked by Wat-1 to the 3'OH of Gal-2. Gln-226 and Asn 186 form hydrogen bonds with the hydroxyl groups of 4'C of Gal-2 and 9'C of Sia-1.

Oligosaccharides
Some Asparagines of HA1 (8, 22, 38, 81, 165, 285)(*) have oligosaccharides chains attached to them. The seventh oligasaccharide is linked to an Aspargine of HA2 (484). All of them are complex oligosaccharides and are on the lateral surfaces of the molecule, except for site and 165. No precise functions have been assigned to them, even if the oligosaccharide at 165 seems to allow the stabilisation of oligomeric contact between globular units at the top of the protein's structure.

Antigenic variability
Each Hemagglutinin monomer contains four antigenic sites called A,B,C and D sites. "A"(in red) site is a loop that protrudes 8 Angström distally from the surface of the target membrane. "B"(in green) site is composed by the external amino acids of one alpha helix and several amino acids of the receptor binding site which contains the sialic acid. "C"(in blue) site is a 60 Angström bulge from the distal extremity of HA. To finish, "D"(in mangenta) site is located in two beta-sheet of the jelly roll of HA1 globular extremity.

The substitutions of amino acids in these sites allow the influenza virus to escape from the immune system and to spread. Major changes in these antigenic regions can produce lethal influenza pandemics, like in 1957.

3D structures of hemagglutinin
3aj5, 3ah1, 3ah2, 3ah4 – CbHA1 + glucoside - Clostridium botulinum

3aj6 - CbHA1 (mutant) + glucoside

2zoe, 2zs6 – CbHA3 + glucoside

2e4m – CbHA1 + HA17

Influenza hemagglutinin
3qqi, 3mlh - IvHA1 receptor-binding domain – Influenza virus

2jrd, 1ibn, 1ibo – IvHA fusion domain – NMR

2dci, 2xoo, 2xop - IvHA fusion domain (mutant) – NMR

2ibx, 1ti8, 2wr0, 2wr1, 2wr2, 2wr3, 2wr4, 2wr5, 2wr7, 2wrb, 2wrc, 2wrd, 2wre, 2wrf, 2wrg, 2wrh – IvHA

1ha0 – IvHA precursor (mutant)

3m5g, 3al4, 3lzg, 3m6s, 3ku3, 3ku5, 3hto, 3eyj, 3bt6, 1ru7, 1ruy, 1ruz, 1rd8, 1mql, 1htm, 2hmg, 3hmg, 4hmg, 5hmg - IvHA1 + HA2

3htp, 3htq, 3htt, 2rft, 2rfu, 1rv0, 1rvt, 1rvx, 1rvz, 1mqm, 1mqn, 1hgd, 1hge, 1hgf, 1hgg, 1hgh, 1hgi, 1hgj, 3m5h, 3m5i, 3m5j - IvHA1 + HA2 + polysaccharide

3eyk, 3eym - IvHA1 + HA2 + membrane-fusion inhibitor

3ku6 - IvHA1 (mutant) + HA2

2viu - IvHA1 (mutant) + HA2 (mutant)

3r2x – IvHA1 receptor-binding domain + HA2 fusion subunit + designed HA-binding protein

3qqb, 3qqe - IvHA1 + HA2 ectodomain (mutant)

2fk0 - IvHA14 receptor-binding domain + HA2 membrane-fusion domain

3lzf, 3gbm, 3gbn, 3fku, 1ken, 1eo8, 1qfu, 2vir - IvHA1 + HA2 + antibody

2vis, 2vit - IvHA1 (mutant) + antibody

Parainfluenza hemagglutinin-neuraminidase
1usx – NvPIV head domain + thiosialoside – Newcastle diasease virus

1usr, 1e8t, 1e8u, 1e8v - NvPIV head domain

1v2i, 2v3b – hPIV – Human parainfluenza virus

2v3c, 2v3d – hPIV + glucoside

2v3e – hPIV + zanamavir

1z4v, 1z4w, 1z4z, 1z50 – SvPIV extracellular domain + DANA – Simian virus

1z4x, 1z4y - SvPIV extracellular domain + glucoside

Measles hemagglutinin
2rkc – MvHA extracellular domain – Measles virus

2zb5, 2zb6 - MvHA head domain

3alw, 3alx, 3alz – MvHA/SLAM receptor

3inb – MvHA head domain + hMembrane cofactor protein

1rwr – HA filamentous – Bordetella pertussis

Dr hemagglutinin
2w5p – EcDrae adhesin subunit – Escherichia coli

1ut1 - EcDrae adhesin subunit (mutant)

2jkj, 2jkl, 2jkn, 1usq - EcDrae adhesin subunit + chloramphenicol derivative

Phytohemagglutinin
1g8w, 1fat – PHA-L – kidney bean

Additional Resources
For additional information, see: Influenza